ABSTRACT
Objective To study the audiology characteristics of infants with human cytomegalovirus (HMCV) pneumonia.Methods Auditory brainstem response (ABR) and acoustic impedance test were carried on the following three groups respectively.The CMV(+) group had 115 infants who have been diagnosed with CMV pneumonia.CMV(-) group had 115 infants with pneumonia but did not have CMV infection.The control Group had 115 healthy children during the same period.A comprehensive evaluation of audiological characteristics and follow-up of hearing changes were studied.Results ①The CMV(+) group had 50 ears (21.74％),the CMV(-) group had 34 ears (14.78％),and the healthy group had 17 ears 7.39％ in ABR V wave threshold over 30 dB nHL.②The CMV(+) group had the highest rate of sens0rineural hearing loss(SNHL) compared with the CMV(-) and health control groups.③The percentages of the abnormal conductive hearing loss(CHL) in the CMV(+) group and CMV(-) group were higher than the health control group while there was no obvious difference between the CMV(+) and the CMV(-) groups.④During a 3-year follow-up,3 infants in the CMV(+)group who suffered SHL recovered while all the infants suffered CHL recovered in hearing.Conclusion Infants with CMV pneumonia have a higher rate of SHL,and pneumonia is likely to cause dysfunction of the middle ear,which might lead to CHL.We need to test infants with CMV pneumonia through thorough clinical audiology examinations and a long-term follow-up.
ABSTRACT
Objective·To investigate the effect of mesenchymal stem cells (MSCs) on autophagy in hippocampus of neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods·Western blotting was used to detect the expression levels of autophagy associated proteins Beclin1, LC3 Ⅱ , and p62 in the hippocampus of HIBD rats following MSCs transplantation and oxygen glucose deprivation (OGD)-injured primary neurons following MSCs seperated coculture. The learning-memory function in the HIBD rats after MSCs transplantation was tested by Morris water maze test. Transmission electron microscopy was also used to observe the number of autophagic neurons in OGD damaged neurons after coculture with MSCs. Results·The levels of Beclin1 and LC3 Ⅱ protein expressions were significantly increased at 12-24 h in the rat hippocampus following HIBD injury. MSCs transplantation statistically downregulated the autophagy level in the hippocampus, and obviously improved the learning-memory function of HIBD rats. Meanwhile, the levels of Beclin1 and LC3 Ⅱ protein expressions in the primary neurons in vitro were also induced by OGD for 12 h. MSCs seperated coculture significantly downregulated the autophagy level of hippocampal neurons by OGD injury, decreased the number of autophagosome in the OGD-injured neurons. Conclusion·MSCs may inhibit the autophagy of hippocampal neurons by partly regulating the damaged microenvironment to improve the learning and memory function of HIBD rats.